Journal: ACS infectious diseases

Article Title: Rapid in vitro assessment of Clostridium difficile inhibition by probiotics using dielectrophoresis to quantify cell structure alterations

doi: 10.1021/acsinfecdis.9b00415

Figure Lengend Snippet: Growth of C. difficile HTCD (A–D) and BI strains (E–H) in supernatants of B. longum, L. acidophilus, L. rhamnosus, L. casei, and S. boulardii supernatants. Absorbance measurements were taken every 20 min for 24 h. The main plots show growth for 24 h, and the respective insets (i–viii) show growth from 0 to 2 h. Solid lines indicate absorbance at 589 nm, while the shaded space around the dotted lines represent errors (SEM values).

Article Snippet: Saccharomyces boulardii (Swanson Health Products, Fargo, ND), Lactobacillus acidophilus (Swanson Health Products), Lactobacillus casei ss. shirtota (Yakult Honsha, Tokyo, Japan), Lactobacillus rhamnosus (Swanson Health Products), and Bifidobacterium longum ss. infantis (Align, Proctor and Gamble, Cincinnati, OH) were isolated from commercial probiotic products.

Techniques:

Journal: ACS infectious diseases

Article Title: Rapid in vitro assessment of Clostridium difficile inhibition by probiotics using dielectrophoresis to quantify cell structure alterations

doi: 10.1021/acsinfecdis.9b00415

Figure Lengend Snippet: Representative images of gram stained HTCD taken at 400× magnification after coculture in supernatants of indicated probiotics: A: HTCD control, B: B. longum, C: S. boulardii, D: L. acidophilus, E: L. casei, F: L. rhamnosus.

Article Snippet: Saccharomyces boulardii (Swanson Health Products, Fargo, ND), Lactobacillus acidophilus (Swanson Health Products), Lactobacillus casei ss. shirtota (Yakult Honsha, Tokyo, Japan), Lactobacillus rhamnosus (Swanson Health Products), and Bifidobacterium longum ss. infantis (Align, Proctor and Gamble, Cincinnati, OH) were isolated from commercial probiotic products.

Techniques: Staining, Probiotics, Control

Journal: ACS infectious diseases

Article Title: Rapid in vitro assessment of Clostridium difficile inhibition by probiotics using dielectrophoresis to quantify cell structure alterations

doi: 10.1021/acsinfecdis.9b00415

Figure Lengend Snippet: TEM images (50 000×) of alterations to the cell wall structure of the HTCD C. difficile strain after 4 h of coculture with supernatants from the indicated probiotic bacteria: A: HTCD control, B: S. boulardii, C: B. longum, D: L. rhamnosus, E: L. acidophilus, F: L. casei. Scale bar is the same across the images and is indicated in A (same for B–F).

Article Snippet: Saccharomyces boulardii (Swanson Health Products, Fargo, ND), Lactobacillus acidophilus (Swanson Health Products), Lactobacillus casei ss. shirtota (Yakult Honsha, Tokyo, Japan), Lactobacillus rhamnosus (Swanson Health Products), and Bifidobacterium longum ss. infantis (Align, Proctor and Gamble, Cincinnati, OH) were isolated from commercial probiotic products.

Techniques: Bacteria, Control

Journal: ACS infectious diseases

Article Title: Rapid in vitro assessment of Clostridium difficile inhibition by probiotics using dielectrophoresis to quantify cell structure alterations

doi: 10.1021/acsinfecdis.9b00415

Figure Lengend Snippet: Summary of Alterations to C. difficile Strains after Coculture in the Indicated Probiotic Supernatants

Article Snippet: Saccharomyces boulardii (Swanson Health Products, Fargo, ND), Lactobacillus acidophilus (Swanson Health Products), Lactobacillus casei ss. shirtota (Yakult Honsha, Tokyo, Japan), Lactobacillus rhamnosus (Swanson Health Products), and Bifidobacterium longum ss. infantis (Align, Proctor and Gamble, Cincinnati, OH) were isolated from commercial probiotic products.

Techniques:

Journal: Immunology

Article Title: Lactobacillus gasseri OLL2809 and its RNA suppress proliferation of CD4 + T cells through a MyD88-dependent signalling pathway

doi: 10.1111/j.1365-2567.2011.03455.x

Figure Lengend Snippet: LG2809 suppressed proliferation of DO11.10 murine splenic CD4+ T cells activated with antigenic stimulation. T-cell-depleted splenocytes from BALB/c mice used as antigen-presenting cells (1 × 105 cells/well) were cultured in the presence or absence of heat-killed Lactobacillus strains, LG2809, MEP225101 and MEP225102 (10 μg dry weight/ml) (a) or indicated doses of heat-killed LG2809 (b) for 4 hr. Subsequently, splenic CD4+ T cells from DO11.10 mice (5 × 104 cells/well) and OVA 323–339 (1 μm) were added to the culture. After 72 hr, the cells were pulsed with [3H]thymidine for 24 hr, and [3H]thymidine incorporation was measured. Data are shown as the mean ± SD. Data are representative of three independent experiments. Statistical differences were analysed by Tukey's tests. Data are statistically different (P < 0.05) among those columns with different symbols.

Article Snippet: LG2809, L. gasseri MEP225101 (MEP225101) and Lactobacillus casei MEP225102 (MEP225102) were isolated by Meiji Dairies Corporation, Japan.

Techniques: Cell Culture

Journal: Immunology

Article Title: Lactobacillus gasseri OLL2809 and its RNA suppress proliferation of CD4 + T cells through a MyD88-dependent signalling pathway

doi: 10.1111/j.1365-2567.2011.03455.x

Figure Lengend Snippet: LG2809 RNA suppressed CD4+ T-cell proliferation from DO11.10 mice. Suppressive effects of bacterial samples were examined as described in the legend to Fig. 1. (a) Heat-killed LG2809 (heat-killed), the homogenate of heat-killed LG2809 (homogenate), the supernatant of the homogenate (sup) and the precipitate of the homogenate (ppt) were added at doses corresponding to 0.1, 1, 10 and 100 μg dry weight LG2809/ml. (b) Five different preparations of the LG2809 homogenate supernatant at a dose corresponding to 100 μg dry weight LG2809/ml were added to the culture. The homogenate supernatant was treated with DNAse I (iii), proteinase K (iv) and RNAse A (v). DNAse I and proteinase K were inactivated by heating at 96° for 10 min, and RNAse A was inactivated with proteinase K treatment. The homogenate supernatants incubated at 37° for 4 hr (i) or at 37° for 4 hr followed by heating at 96° for 10 min (ii) were used as controls. (c) LG2809 RNA (at 0.1, 1 and 10 μg/ml) or (d) RNA from murine splenic cells (at 1 and 10 μg/ml) were added to the culture. Data are shown as the mean ± SD. Data are representative of three independent experiments. Statistical differences were analysed by Tukey's tests. Data are statistically different (P < 0.05) among those with different symbols.

Article Snippet: LG2809, L. gasseri MEP225101 (MEP225101) and Lactobacillus casei MEP225102 (MEP225102) were isolated by Meiji Dairies Corporation, Japan.

Techniques: Incubation

Journal: Immunology

Article Title: Lactobacillus gasseri OLL2809 and its RNA suppress proliferation of CD4 + T cells through a MyD88-dependent signalling pathway

doi: 10.1111/j.1365-2567.2011.03455.x

Figure Lengend Snippet: LG2809 and its RNA suppressed proliferation of CD4+ T cells stimulated with anti-CD3/CD28 treatment in the absence of antigen-presenting cells. Plates were first coated with 10 μg/ml anti-CD3ε monoclonal antibody (mAb) for 2 hr at 37°. Subsequently, splenic CD4+ T cells from BALB/c mice (5 × 104 cells/well) and 2 μg/ml anti-CD28 mAb were added with indicated doses of heat-killed LG2809 (a), LG2809 RNA (b) or RNA from murine splenic cells (c) and were cultured for 72 hr. The cells were pulsed with [3H]thymidine for 24 hr, and [3H]thymidine incorporation was measured. Data are shown as the mean ± SD. Data are representative of three independent experiments. Statistical differences were analysed using Tukey's tests. Data are statistically different (P < 0.05) among those with different symbols.

Article Snippet: LG2809, L. gasseri MEP225101 (MEP225101) and Lactobacillus casei MEP225102 (MEP225102) were isolated by Meiji Dairies Corporation, Japan.

Techniques: Cell Culture

Journal: Immunology

Article Title: Lactobacillus gasseri OLL2809 and its RNA suppress proliferation of CD4 + T cells through a MyD88-dependent signalling pathway

doi: 10.1111/j.1365-2567.2011.03455.x

Figure Lengend Snippet: Suppressive effect of LG2809 on proliferation of CD4+ T cells was mediated through a MyD88-dependent signalling pathway. Splenic CD4+ T cells (5 × 104 cells/well) from wild-type (a) or MyD88-deficient BALB/c mice (b) were stimulated with plate-bound anti-CD3ε and soluble anti-CD28 monoclonal antibodies, as described in the legend to Fig. 3, in the presence of heat-killed LG2809 (10 μg/ml) or LG2809 RNA (10 μg/ml). Proliferation of the cells was measured as described. Data are shown as the mean ± SD. Data are representative of three independent experiments. Statistical differences were analysed using Tukey's tests. Data are statistically different (P < 0.05) among those with different symbols.

Article Snippet: LG2809, L. gasseri MEP225101 (MEP225101) and Lactobacillus casei MEP225102 (MEP225102) were isolated by Meiji Dairies Corporation, Japan.

Techniques: Bioprocessing

Journal: Immunology

Article Title: Lactobacillus gasseri OLL2809 and its RNA suppress proliferation of CD4 + T cells through a MyD88-dependent signalling pathway

doi: 10.1111/j.1365-2567.2011.03455.x

Figure Lengend Snippet: Reactive oxygen species are generated in CD4+ T cells treated with LG2809 and its RNA and their suppressive effect is abrogated by antioxidant treatment. (a) Splenic CD4+ T cells from BALB/c mice (1 × 105 cells/well) were stimulated with plate-bound anti-CD3ε and soluble anti-CD28 monoclonal antibodies, as described in the legend to Fig. 3, in the presence of heat-killed LG2809 (10 μg/ml) or LG2809 RNA (10 μg/ml) with or without N-acetyl-cysteine (NAC) at 25 mm. Proliferation of the cells was measured as described. Data are shown as the mean ± SD and are representative of three independent experiments. (b, c) Splenic CD4+ T cells from BALB/c mice (1 × 105 cells/well) were cultured in the presence of heat-killed LG2809 (10 μg/ml) or LG2809 RNA (10 μg/ml) for 1 hr with or without NAC at 25 mm. After the incubation, all groups of the cells were stained with 25 mm 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) for 30 min and analysed by flow cytometry. The open histograms indicate the cells incubated with LG2809 or LG2809 RNA and the shaded histograms indicate the cells cultured with the medium alone. (c) Data are shown as the mean ± SD of mean fluorescence intensities of triplicate culture and are representative of three independent experiments. Statistical differences were analysed by Student's t-tests. NS = not significant, *P < 0.05, and **P < 0.01.

Article Snippet: LG2809, L. gasseri MEP225101 (MEP225101) and Lactobacillus casei MEP225102 (MEP225102) were isolated by Meiji Dairies Corporation, Japan.

Techniques: Generated, Bioprocessing, Cell Culture, Incubation, Staining, Flow Cytometry, Fluorescence

Journal: Immunology

Article Title: Lactobacillus gasseri OLL2809 and its RNA suppress proliferation of CD4 + T cells through a MyD88-dependent signalling pathway

doi: 10.1111/j.1365-2567.2011.03455.x

Figure Lengend Snippet: LG2809 RNA suppressed the delayed-type hypersensitivity response. DO11.10 mice were immunized with ovalbumin (OVA) and complete Freund's adjuvant (CFA) at the base of the tail. After 14 days, saline was injected into the left footpad, and saline containing OVA with or without the sample was injected into the right footpad. After 24 hr, the footpad swelling was measured and calculated using the following formula: the thickness of right footpad (mm) – the thickness of left footpad (mm). Data are shown as the mean ± SD. Data are representative of three independent experiments. Statistical differences were analysed using a Tukey's test. Data are statistically different (P < 0.05) among those with different symbols.

Article Snippet: LG2809, L. gasseri MEP225101 (MEP225101) and Lactobacillus casei MEP225102 (MEP225102) were isolated by Meiji Dairies Corporation, Japan.

Techniques: Adjuvant, Saline, Injection

Journal: Drugs in Context

Article Title: New and emerging oral therapies for psoriasis

doi: 10.7573/dic.2024-5-6

Figure Lengend Snippet: Oral microbial therapy studies on efficacy and adverse effects of oral therapies in psoriasis.

Article Snippet: KBL697 is an oral probiotic developed by the South Korean biotechnology company KoBioLabs from a strain of the bacterium Lactobacillus gasseri , which, unlike EDP1815, is a live agent, directly altering the gut microbiome.

Techniques: Clinical Proteomics

Journal: BMC Infectious Diseases

Article Title: Role of probiotic as adjuvant in treating various infections: a systematic review and meta-analysis

doi: 10.1186/s12879-024-09259-3

Figure Lengend Snippet: Table of study characteristics

Article Snippet: Laue et al., 2017 [ ] , II , Randomiezed double blind , Bad Segeber, Germany , 48 participants with BV diagnosed by Nugent score Intervention group: - mean age ± SD: 32.6 ± 11.2 - 100% were females Placebo group: - mean age ± SD: 39.0 ± 12.3 - 100% were females , 4 weeks-Metronidazole with Verum (yogurt contains L. crispatus, L. gasseri, L. rhamnosus, and L. jensenii with a total 1 × 10 7 CFU/mL) , 2 yogurt drinks daily or as a placebo The placebo treatment consisted of daily 2 × 125 g chemically (with H 3 PO 4 ) acidified milk without bacterial strains , 4 weeks after the probiotic treatment , The parameter used is Nugent score 0–3 , I: 81.3% P: 64.7% p -value: 0.438 (NS).

Techniques: Capsules, Control, Adjuvant, Infection, Probiotics, Protein-Protein interactions, Functional Assay, Maltodextrin, Formulation, Staining, Virus, Cell Counting